Active zones of mammalian neuromuscular junctions: formation, density, and aging
نویسندگان
چکیده
منابع مشابه
Spontaneous acetylcholine release in mammalian neuromuscular junctions.
Spontaneous secretion of the neurotransmitter acetylcholine in mammalian neuromuscular synapsis depends on the Ca2+ content of nerve terminals. The Ca2+ electrochemical gradient favors the entry of this cation. We investigated the possible involvement of three voltage-dependent Ca2+ channels (VDCC) (L-, N-, and P/Q-types) on spontaneous transmitter release at the rat neuromuscular junction. Min...
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Formation of neuromuscular junctions in embryonic cultures has been reviewed. Anatomical and electrophysiological evidence has been presented which demonstrates the formation de novo of synaptic contacts between nerve and muscle in culture; in (1) organ cultures of spinal cord explanted intact with attached myotomes, (2) organ cultures of in vitro coupled explants of spinal cord and skeletal mu...
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In this study we established a transgenic Ca2+ imaging technique in Drosophila that enabled us to target the Ca2+ sensor protein yellow Cameleon-2 specifically to larval neurons. This noninvasive method allowed us to measure evoked Ca2+ signals in presynaptic terminals of larval neuromuscular junctions (NMJs). We combined transgenic Ca2+ imaging with electrophysiological recordings and morpholo...
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The polyene antibiotic filipin reacts specifically with membrane cholesterol and produces distinctive membrane lesions. We treated frog cutaneous and sartorius muscles with 0.04% filipin in a glutaraldehyde solution with or without prefixation with glutaraldehyde. Freeze-fracture of these muscles revealed numerous 19 to 38-nm protuberances and depressions (filipin-sterol complexes) in most area...
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The purpose of this study has been to examine, by means of isolation in vitro, the conditions under which neuromyal junctions develop. More than a hundred years ago Doyere (1840) observed in the water-bear Milnesium tardigradum that the nerve fibers terminate in characteristic eminences of muscle fibers. This observation, made on an arthropod, stimulated research into the connexion between musc...
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ژورنال
عنوان ژورنال: Annals of the New York Academy of Sciences
سال: 2012
ISSN: 0077-8923
DOI: 10.1111/j.1749-6632.2012.06836.x